Bacterial Immunoabsorbent Columns

Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A.

Earlier attempts to express peptides longer than 20 aa on the surface of tobamoviruses such as tobacco mosaic virus have failed. Surprisingly, we found that a functional fragment of protein A (133 aa) can be displayed on the surface of a tobamovirus as a C-terminal fusion to the coat protein via a 15-aa linker. The macromolecular nature of these nanoparticles allowed the design of a simple prot...

Cortical Columns

The most prominent feature of the architecture of the cortex is its horizontal organization into layers. Each layer contains different cell types, and forms different types of connections with other neurons. However, a strong vertical organization is often also apparent: neurons stacked on top of each other through the depth of the cortex tend to be connected and have similar response propertie...

HPLC Columns

BACTERIAL TRANSPORT IN LABORATORY COLUMNS AND FILTERS: INFLUENCE OF IONIC STRENGTH AND pH ON COLLISION EFFICIENCY

-The influence of ionic strength and pH on the transport of Pseudomonasfluorescens PI7 in porous media was investigated using continuous-flow laboratory columns and a rapid screening technique in which radiolabeled cells were applied to large-pore, glass-fiber filters. Colloid-filtration theory was used to interpret P17 transport results in the two systems. Bacterial retention was directly rela...

Silicate entrapped columns--new columns designed for capillary electrochromatography.

Designed especially for capillary electrochromatography (CEC), silicate-entrapped columns are made by trapping particles of chromatographic packing material in a network of silica. Once entrapped, the capillary no longer requires frits. This renders a more homogeneous and stable packed bed. Accidental breakage of the fragile frits is not an issue with these robust columns. Columns packed with r...